Journal:

Article Title: Choroidal neovascular membrane inhibition in a laser treated rat model with intraocular sustained release triamcinolone acetonide microimplants

doi:

Figure Lengend Snippet: Microimplant designs. The control implant (20% PVA only, far left), implant A (8.62% TAAC matrix, left centre), implant B (3.62% TAAC matrix, right centre), and implant C (dual 8.62% TAAC matrix with a 200 μg TAAC central reservoir, far right). The matrix for each design consists of 20% polyvinyl alcohol (PVA).

Article Snippet: Twenty male adult (250 g) Brown Norway rats (Harlan Sprague Dawley, Inc, Indianapolis, IN, USA) were used to evaluate the effect of intravitreal TAAC microimplants versus control implants on FVP.

Techniques: Control

Journal:

Article Title: Choroidal neovascular membrane inhibition in a laser treated rat model with intraocular sustained release triamcinolone acetonide microimplants

doi:

Figure Lengend Snippet: Cumulative mean release of TAAC (μg (1 SD)) versus the square root of time from implants A and B.

Article Snippet: Twenty male adult (250 g) Brown Norway rats (Harlan Sprague Dawley, Inc, Indianapolis, IN, USA) were used to evaluate the effect of intravitreal TAAC microimplants versus control implants on FVP.

Techniques:

Journal:

Article Title: Choroidal neovascular membrane inhibition in a laser treated rat model with intraocular sustained release triamcinolone acetonide microimplants

doi:

Figure Lengend Snippet: Cumulative mean release of TAAC (μg (1 SD)) versus time from implant C.

Article Snippet: Twenty male adult (250 g) Brown Norway rats (Harlan Sprague Dawley, Inc, Indianapolis, IN, USA) were used to evaluate the effect of intravitreal TAAC microimplants versus control implants on FVP.

Techniques:

Journal:

Article Title: Choroidal neovascular membrane inhibition in a laser treated rat model with intraocular sustained release triamcinolone acetonide microimplants

doi:

Figure Lengend Snippet: Comparison of mean FVP thickness in eyes receiving TAAC microimplants versus eyes receiving control implants

Article Snippet: Twenty male adult (250 g) Brown Norway rats (Harlan Sprague Dawley, Inc, Indianapolis, IN, USA) were used to evaluate the effect of intravitreal TAAC microimplants versus control implants on FVP.

Techniques: Comparison, Control

Journal:

Article Title: Choroidal neovascular membrane inhibition in a laser treated rat model with intraocular sustained release triamcinolone acetonide microimplants

doi:

Figure Lengend Snippet: Representative histology (radial view) of four untreated control and four TAAC treated lesion sites at 35 days following lesion induction and placement of control versus TAAC microimplants, or of control versus TAAC intravitreal injections. Tissues were stained with haematoxylin and eosin. The scale bar in each photograph represents 30 μm. (A) PVA only microimplant control (fellow eye to implant A). Note the FVP (fibrovascular proliferation) arising through the disrupted retinal pigment epithelium (RPE) and Bruch’s membrane and infiltrating the retina. Well stained erythrocytes can be observed within lumenal structures. (B) Implant A, 8.62% (w/v) TAAC treated. Note the prominent defects in the RPE and Bruch’s membrane with a striking inhibition of FVP. The retina is drawn into the defect. (C) PVA only microimplant control (fellow eye to implant B). Note the FVP arising through the disrupted RPE and Bruch’s membrane and infiltrating the retina. (D) Implant B, 3.62% (w/v) TAAC treated. Note the prominent defects in the RPE and Bruch’s membrane with a striking inhibition of FVP. The retina is drawn into the defect, similar to the lesions from the eyes treated with the implant A design. (E) PVA only microimplant control (fellow eye to implant C). Note the FVP arising through the disrupted RPE and Bruch’s membrane with infiltration into the proximal retina, similar to the control lesions from the other control eyes. (F) Implant C, 8.62% (w/v) TAAC matrix with central TAAC reservoir treated. Note the inhibition of FVP on the left side of defect, with partial FVP development on the right side. (G) Intravitreal injection control (20 μl physiological saline). Similar to the other control eyes, note the FVP arising from the laser induced defect in the choroid and RPE with extensive retinal infiltration. (H) TAAC intravitreal injection treated (0.8 mg). Note the marked inhibition of FVP, similar to the effect produced in eyes receiving TAAC microimplants.

Article Snippet: Twenty male adult (250 g) Brown Norway rats (Harlan Sprague Dawley, Inc, Indianapolis, IN, USA) were used to evaluate the effect of intravitreal TAAC microimplants versus control implants on FVP.

Techniques: Control, Staining, Membrane, Inhibition, Injection, Saline, Produced

Journal: Plant Physiology

Article Title: Role of Thylakoid ATP/ADP Carrier in Photoinhibition and Photoprotection of Photosystem II in Arabidopsis ^{1 [W] [OA]}

doi: 10.1104/pp.110.155804

Figure Lengend Snippet: Comparison of the taac mutant with wild-type Arabidopsis plants of the same Ws ecotype. A, The photographs of representative plants were taken at an age of 42 d of growth using a hydroponic system at an irradiance of 120 μmol photons m−2 s−1. B, Plot of shoot weight ± sd as a function of plant age (n = 10). The difference in the weight is significant at 6 and 8 weeks of growth (Student's t test P < 0.05). C, Transmission electron microscopy images, showing chloroplast ultrastructure, were recorded at an age of 28 d of growth. Bar = 2 μm.

Article Snippet: A second homozygous taac mutant identified from Syngenta T-DNA insertion lines collection (SAIL_209_A12, Columbia ecotype) is described in Supplemental Figure S2.

Techniques: Mutagenesis, Transmission Assay, Electron Microscopy

Journal: Plant Physiology

Article Title: Role of Thylakoid ATP/ADP Carrier in Photoinhibition and Photoprotection of Photosystem II in Arabidopsis ^{1 [W] [OA]}

doi: 10.1104/pp.110.155804

Figure Lengend Snippet: Pigment composition and xanthophyll cycle in Ws ecotype plants and the taac mutant. A, Leaves from plants grown at an irradiance of 120 μmol photons m−2 s−1 were detached at the end of the dark period, and the pigment composition was determined by HPLC. Amounts of main pigments are expressed relative to Chl a ± sd (n = 17). B, Leaves were illuminated with PAR of intensities ranging between 53 and 1,952 μmol photons m−2 s−1. The time of illumination was 20 min for PAR intensities <1,000 μmol photons m−2 s−1 and reduced to 10 min for higher PAR intensities. Following illumination, pigments were extracted and analyzed by HPLC. Activity of the xanthophyll cycle was determined as de-epoxidation ratio (DR) ± sd (n = 3–4 for each point). DR = (antheraxanthin + zeaxanthin)/(antheraxanthin + zeaxanthin + violaxanthin). C, Effect of nigericin and DTT on the kinetics of NPQ formation. Prior recording kinetics as described in Figure 2, plants were vacuum infiltrated with 50 μm nigericin (NGC) or 5 mm DTT. The curves for control leaves (infiltrated with water) were similar to those presented in Figure 2 and therefore are not shown here.

Article Snippet: A second homozygous taac mutant identified from Syngenta T-DNA insertion lines collection (SAIL_209_A12, Columbia ecotype) is described in Supplemental Figure S2.

Techniques: Mutagenesis, Activity Assay

Journal: Plant Physiology

Article Title: Role of Thylakoid ATP/ADP Carrier in Photoinhibition and Photoprotection of Photosystem II in Arabidopsis ^{1 [W] [OA]}

doi: 10.1104/pp.110.155804

Figure Lengend Snippet: Comparison of the taac mutant with wild-type Arabidopsis plants of the same Ws ecotype. A, The photographs of representative plants were taken at an age of 42 d of growth using a hydroponic system at an irradiance of 120 μmol photons m−2 s−1. B, Plot of shoot weight ± sd as a function of plant age (n = 10). The difference in the weight is significant at 6 and 8 weeks of growth (Student's t test P < 0.05). C, Transmission electron microscopy images, showing chloroplast ultrastructure, were recorded at an age of 28 d of growth. Bar = 2 μm.

Article Snippet: Arabidopsis seeds for the SAIL_209_A12 T-DNA insertion line for the TAAC gene (Columbia ecotype; Supplemental Fig. S2A) were produced by Syngenta ( McElver et al., 2001 ) and ordered at the Salk SIGnAL T-DNA express Arabidopsis Gene mapping tool ( http://www.signal.salk.edu/cgi-bin/tdnaexpress ).

Techniques: Comparison, Mutagenesis, Transmission Assay, Electron Microscopy

Journal: Plant Physiology

Article Title: Role of Thylakoid ATP/ADP Carrier in Photoinhibition and Photoprotection of Photosystem II in Arabidopsis ^{1 [W] [OA]}

doi: 10.1104/pp.110.155804

Figure Lengend Snippet: Photosynthetic characteristics of wild-type Arabidopsis Ws ecotype and taac plants grown at an irradiance of 120 μ mol photons m −2 s −1 The parameters measured on 42-d-old plants were expressed as means ± sd . *, Significantly different from the wild type (Student's t test P = 0.001).

Article Snippet: Arabidopsis seeds for the SAIL_209_A12 T-DNA insertion line for the TAAC gene (Columbia ecotype; Supplemental Fig. S2A) were produced by Syngenta ( McElver et al., 2001 ) and ordered at the Salk SIGnAL T-DNA express Arabidopsis Gene mapping tool ( http://www.signal.salk.edu/cgi-bin/tdnaexpress ).

Techniques: